What purity should NAD+ have?

The research-grade purity standard

For research-grade peptides like NAD+, the gold standard is 99%+ HPLC-verified purity. HPLC (high-performance liquid chromatography) separates a peptide sample into its component molecules and measures the relative proportion of the target peptide versus any impurities, degradation products, or by-products from synthesis.

A 99%+ result means that less than 1% of the sample by mass is anything other than the target NAD+ molecule. For experimental reproducibility, anything below 95% introduces significant uncertainty — your results may be influenced by impurities rather than the peptide itself.

How to read a Certificate of Analysis (COA)

A real COA for NAD+ should contain:

1. HPLC chromatogram — a visual trace showing peaks that correspond to the peptide's retention time
2. Purity percentage — a specific number (e.g. 99.3%) derived from integrating the chromatogram
3. Mass spectrometry confirmation — a molecular-weight reading matching the theoretical mass of NAD+
4. Batch/lot number — tied to a specific manufacturing run
5. Testing lab + date — independent third party, not self-certified
6. Expiry date — based on stability studies for the compound

If any of these are missing, you cannot rely on the purity claim. Every Peptify COA includes all of the above and is downloadable from the product page.

Why impurities matter in research

Even small amounts of impurities in a research peptide can bind to unintended targets, trigger off-target effects in cell culture, or confound measurement assays. For quantitative research (dose-response curves, binding kinetics, concentration-dependent effects), using a <99% pure peptide can invalidate your data entirely.

Buy NAD+ for research

99%+ HPLC-verified NAD+ in stock. Every vial ships from the UK with a batch-specific Certificate of Analysis. Same-day dispatch on orders before 1pm.

• NAD 500MG

Frequently asked questions