Common mistakes with LL-37

Mistake 1: Improper storage after reconstitution

Once LL-37 is reconstituted with bacteriostatic water, it must be stored at 2-8°C (refrigerated) and used within 28 days. A common mistake is leaving the vial at room temperature for extended periods, which degrades the peptide and can invalidate experimental results.

Freeze-thaw cycles are another issue — each cycle reduces peptide integrity. If your protocol requires long-term storage, keep the lyophilised vial frozen at -20°C until the moment of reconstitution.

Mistake 2: Concentration miscalculation

Calculating the target concentration wrong is one of the most common experimental errors with LL-37. Always double-check:

• The stated vial mass (e.g. 5mg, 10mg)
• The volume of bacteriostatic water added
• The resulting concentration (mass / volume)
• The volume of reconstituted solution you draw into the syringe for each dose point

A simple spreadsheet or lab notebook with these values at the top of every experiment can prevent costly errors.

Mistake 3: Skipping COA verification

Researchers sometimes trust a supplier's purity claim without checking the actual COA. For LL-37, always:

1. Match the batch number on your vial to the COA on the product page
2. Verify the HPLC chromatogram shows a single clean peak
3. Confirm the mass spectrometry reading matches the theoretical molecular weight
4. Check the expiry date

Skipping this step means you are treating purity as a marketing claim rather than a verifiable fact. Peptify publishes every batch COA at peptifyuk.com, downloadable directly from each product page.

Buy LL-37 for research

99%+ HPLC-verified LL-37 in stock. Every vial ships from the UK with a batch-specific Certificate of Analysis. Same-day dispatch on orders before 1pm.

• LL 37 5MG

Frequently asked questions