Independent peptide testing labs explained
How third-party HPLC peptide testing works, what a real Certificate of Analysis contains, and which independent laboratories UK research peptide suppliers cite for batch verification.
What is third-party COA testing?
A Certificate of Analysis (COA) is a document issued by an analytical laboratory that records the test results for a specific batch of material. For research peptides, a COA typically contains an HPLC chromatogram, a purity percentage derived from integrating the chromatogram, a mass-spectrometry reading confirming the molecular weight, a batch or lot number, and the testing laboratory's name and signature.
Third-party means the laboratory is independent of the manufacturer — not in-house QC. The independence is the value: the laboratory has no commercial incentive to inflate the purity reading.
How Janoshik Analytical fits in
Janoshik Analytical is an independent analytical laboratory in the Czech Republic providing HPLC purity testing and mass-spectrometry identity confirmation for research peptides. Janoshik publishes a public COA portal at public.janoshik.com and is the laboratory Peptify UK uses for every production lot.
HPLC, mass spectrometry, UV — the three core methods
- HPLC (high-performance liquid chromatography) — separates a sample into its component molecules and measures the relative proportion of the target peptide versus impurities. The dominant purity-percentage method for research peptides.
- Mass spectrometry — ionises eluting molecules and measures their mass-to-charge ratio. Confirms whether the molecular weight of the eluting peak matches the theoretical mass of the target peptide.
- UV detection — the in-line detector on most HPLC systems. Compounds absorb UV light at characteristic wavelengths (typically 214 nm for peptide bonds); the absorbance signal produces the chromatogram peaks.
Why batch-by-batch testing matters
Each synthesis run can produce a different purity profile. A supplier that publishes a single generic certificates page (rather than the lot-specific COA for your vial) is telling you nothing about the actual material in your hands. Per-batch testing closes the reproducibility gap, the identity-confirmation gap, and the stability-anchor gap.
Other independent laboratories UK suppliers cite
- Janoshik Analytical — Czech Republic, HPLC + mass spectrometry, public COA portal. Peptify UK's chosen lab.
- Eurofins — large global analytical-testing group with HPLC and mass-spectrometry services.
- PeptideVerify — US-based independent peptide-analytical laboratory.
- JR Labs — independent analytical laboratory.
Peptify uses Janoshik
Every Peptify production lot is HPLC-tested by Janoshik Analytical before any vial is released. The lot-specific Certificate of Analysis is published on the corresponding product page. Browse published COAs at /certificates/.
For research use only. Not for human consumption. PEPTIFY LIMITED #17021295.